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pegfp nfat 3 expression plasmid  (Addgene inc)


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    Structured Review

    Addgene inc pegfp nfat 3 expression plasmid
    Pegfp Nfat 3 Expression Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+nfat+3+expression+plasmid/pEGFP-C1+NFAT3+(Plasmid+%2310961)/pmc02707217-51-1-8
    Average 93 stars, based on 18 article reviews
    pegfp nfat 3 expression plasmid - by Bioz Stars, 2026-11
    93/100 stars

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    Expressing:

    Article Title: NFAT-3 Is a Transcriptional Repressor of the Growth-associated Protein 43 during Neuronal Maturation
    Article Snippet: The region of the GAP-43 promoter containing a putative NFAT binding site (see A ) was cloned by PCR and ligated into the HindIII and XhoI restriction sites of a luciferase reporter derived from the NFAT/AP-1 3× luciferase reporter (Addgene, plasmid 11783). .. The pEGFP-NFAT-3 expression plasmid was also obtained from Addgene (plasmid 10961). fig ft0 fig mode=article f1 FIGURE 1. caption a4 NFAT-3 represses GAP-43 expression in PC-12 and cortical neurons. ..

    Article Title: NFAT-3 Is a Transcriptional Repressor of the Growth-associated Protein 43 during Neuronal Maturation
    Article Snippet: Reporter and Expression Plasmid Construct—The region of theGAP-43 promoter containing a putative NFAT binding site (see Fig. 1A) was cloned by PCR and ligated into theHindIII and XhoI restriction sites of a luciferase reporter derived from the NFAT/AP-1 3 luciferase reporter (Addgene, plasmid 11783). .. The pEGFP-NFAT-3 expression plasmid was also obtained from Addgene (plasmid 10961). .. Transfection—Either experiments were performed using Lipofectamine 2000 (Invitrogen) for PC-12 and rat cortical neurons 5 days in vitro (DIV 5), or electroporation with the rat neuron nucleofector kit (Amaxa Biosystems) was used according to the provided protocol.

    Article Title: NFAT-3 Is a Transcriptional Repressor of the Growth-associated Protein 43 during Neuronal Maturation
    Article Snippet: Luciferase Assays Reporter and Expression Plasmid Construct The region of the GAP-43 promoter containing a putative NFAT binding site (see A ) was cloned by PCR and ligated into the HindIII and XhoI restriction sites of a luciferase reporter derived from the NFAT/AP-1 3× luciferase reporter (Addgene, plasmid 11783). .. The pEGFP-NFAT-3 expression plasmid was also obtained from Addgene (plasmid 10961). ..

    Plasmid Preparation:

    Article Title: NFAT-3 Is a Transcriptional Repressor of the Growth-associated Protein 43 during Neuronal Maturation
    Article Snippet: The region of the GAP-43 promoter containing a putative NFAT binding site (see A ) was cloned by PCR and ligated into the HindIII and XhoI restriction sites of a luciferase reporter derived from the NFAT/AP-1 3× luciferase reporter (Addgene, plasmid 11783). .. The pEGFP-NFAT-3 expression plasmid was also obtained from Addgene (plasmid 10961). fig ft0 fig mode=article f1 FIGURE 1. caption a4 NFAT-3 represses GAP-43 expression in PC-12 and cortical neurons. ..

    Article Title: NFAT-3 Is a Transcriptional Repressor of the Growth-associated Protein 43 during Neuronal Maturation
    Article Snippet: Reporter and Expression Plasmid Construct—The region of theGAP-43 promoter containing a putative NFAT binding site (see Fig. 1A) was cloned by PCR and ligated into theHindIII and XhoI restriction sites of a luciferase reporter derived from the NFAT/AP-1 3 luciferase reporter (Addgene, plasmid 11783). .. The pEGFP-NFAT-3 expression plasmid was also obtained from Addgene (plasmid 10961). .. Transfection—Either experiments were performed using Lipofectamine 2000 (Invitrogen) for PC-12 and rat cortical neurons 5 days in vitro (DIV 5), or electroporation with the rat neuron nucleofector kit (Amaxa Biosystems) was used according to the provided protocol.

    Article Title: NFAT-3 Is a Transcriptional Repressor of the Growth-associated Protein 43 during Neuronal Maturation
    Article Snippet: Luciferase Assays Reporter and Expression Plasmid Construct The region of the GAP-43 promoter containing a putative NFAT binding site (see A ) was cloned by PCR and ligated into the HindIII and XhoI restriction sites of a luciferase reporter derived from the NFAT/AP-1 3× luciferase reporter (Addgene, plasmid 11783). .. The pEGFP-NFAT-3 expression plasmid was also obtained from Addgene (plasmid 10961). ..



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